Journal: International Journal of Cardiology. Cardiovascular Risk and Prevention

Article Title: Effects of PCSK9 inhibitors on vascular function, lipid profile, and cardiovascular outcomes in patients with peripheral artery disease: A systematic review and meta-analysis

doi: 10.1016/j.ijcrp.2026.200590

Figure Lengend Snippet: Summary of included studies.

Article Snippet: Clavijoa, 2023 , RCT, Double-blind , USA , 6 months , Evolocumab 420 mg SC q4w , placebo , 35 , 35 , 70 , Patients aged 40–85 years with a diagnosis of atherosclerotic cardiovascular disease and PAD (Rutherford class I-VI), confirmed by an ankle-brachial index (ABI) ≤0.9 at rest or ≤0.8 after exercise, angiography, duplex ultrasound, or a history of lower extremity surgical or endovascular revascularization. Patients had to be at least one month from their most recent intervention, stable on maximal tolerated lipid-lowering therapy for at least four weeks, and have a fasting LDL cholesterol ≥55 mg/dL or non-HDL cholesterol ≥80 mg/dL. , MWT at 6 months , After six months, Evolocumab significantly improved MWT, FMD, Carotid IMT, and plasma MRP-14 levels. In contrast, changes in pain-free walking time, ABI, TBI, transcutaneous oxygen pressure, oxLDL, and sCD36 were not statistically significant..

Techniques: Control, Biomarker Discovery, Clinical Proteomics, Activation Assay

Journal: Journal of Immunology Research

Article Title: High Fat Diet‐Induced Obesity Alters Cutaneous Immune Cell Function, and These Changes Persist After Weight Loss

doi: 10.1155/jimr/3930910

Figure Lengend Snippet: Establishment of the lean, obese, weight loss (WL) groups. (a) The schedules for generating mouse models. (b) Representative photographs of mice from each group after 18 weeks of feeding. (c) Body weight and rate of weight gain. The vertical dotted lines indicate the timing of diet switching ( n = 24 mice per group). (d) Intraperitoneal glucose tolerance test (ipGTT) at 18 weeks post‐diet initiation ( n = 12 mice per group). (e) Representative histological images of eWAT. Arrows indicate crown‐like structures. (f) Serum levels of total cholesterol, LDL cholesterol, and HDL cholesterol ( n = 8 mice per group). Results are representative of at least two independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001, Lean vs. Obese; ## p < 0.01, Lean vs. WL; $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 Obese vs. WL.

Article Snippet: Serum levels of total cholesterol, LDL cholesterol, and HDL cholesterol were measured by Oriental Yeast Co., Ltd.

Techniques:

Journal: Gastroenterology

Article Title: A Gut-Restricted Liver X Receptor Agonist Ameliorates Liver Injury in Experimental Short Bowel Syndrome

doi: 10.1053/j.gastro.2025.12.015

Figure Lengend Snippet: Aggravated IFALD following the deficiency of intestinal ApoA1 and HDL-C after SBR. ( A–D ) WT male ( filled circles ) or female ( open circles ) mice underwent sham or SBR. Starting 3 weeks later, mice received daily vehicle or WUSTL0717 (30 mg/kg, PO) for 7 weeks, followed by analysis (n = 5–9/group). ( A ) Heatmap of cholesterol efflux–related transcripts in the duodenum and post-anastomosis ileum from male mice (n = 3–4/group). ( B ) qRT-PCR analysis of Apoa1 transcripts in the duodenum, post-anastomosis ileum, and liver (n = 4–9/group). ( C and D ) Portal venous HDL-C ( C ) and ApoA1 ( D ) levels from males (plasma) and females (serum) (n = 5–9/group). ( E and F ) Correlation of portal venous plasma HDL-C and ApoA1 levels with liver collagen area ( , upper ). Each dot represents a matched individual from ( C ) and ( D ). ( G–M ) Apoa1 ΔIEC mice underwent SBR and were euthanized 11 weeks later. Dark dots represent males and light dots represent females (males, n = 7/group; females: n = 6–7/group). ( G and H ) Portal venous serum HDL-C and ApoA1 levels. ( I ) Systemic (inferior vena cava) serum ALT levels. ( J ) Liver Col1a1 transcript levels analyzed by qRT-PCR. ( K ) Sirius Red–stained liver sections ( scale bar , 200 μ m) with quantification from 5 to 6 areas per liver, 1 image per mouse (males, n = 7/group; females: n = 6–7/group). ( L and M ) Correlation of portal venous serum HDL-C and ApoA1 levels with liver collagen area. Each dot represents a matched individual from ( K )–( M ). Statistical evaluations were done using unpaired Student t test ( C , G–K ), 1-way analysis of variance with Tukey’s honestly significant difference ( B , D ), or Pearson correlation ( E , F , L , and M ).

Article Snippet: HDL-cholesterol (HDL-C) levels were measured using the HDL-C assay kit (STA-394; Cell Biolabs), and ApoA1 levels by enzyme-linked immunosorbent assay (3750–1HP; Mabtech).

Techniques: Quantitative RT-PCR, Clinical Proteomics, Staining